TRANSGENIC ANIMALS FOR BIOTECHNOLOGY
- Overview
- Assessment methods
- Learning objectives
- Contents
- Delivery method
- Teaching methods
- Contacts/Info
Basic knowledge of Animal Biology
Learning outcomes are assessed through an oral examination designed to evaluate students' understanding and mastery of the expected learning outcomes.
The final oral exam consists of three questions: two focused on the theoretical portion and one on laboratory instruction. The theoretical questions account for 75% of the final grade, while the laboratory-related question assesses students' comprehension of key practical techniques demonstrated during lab sessions.
The exam is graded on a 30-point scale, considering the accuracy and depth of responses, clarity of communication, and the ability to justify statements and opinions effectively. A minimum score of 18/30 is required to pass.
This course provides students with comprehensive knowledge of the diverse applications of biotechnology in livestock production, including the development of genetically modified animals for diagnostic, therapeutic, and environmental protection purposes.
Students will explore both classical and modern biotechnological techniques used to enhance product quality and obtain bioactive compounds. A key focus will be on genetic engineering methods that introduce genes encoding pharmacologically relevant proteins into the DNA of farm animals. Additionally, the course will delve into the production and quality of animal-derived foods and their derivatives.
To complement theoretical learning, students will participate in field trips to observe real-world applications in production and research settings. These experiences will provide valuable insights and opportunities to engage with experts in the field.
Course Overview
This course explores key biotechnological advancements and their applications in animal production, with a particular focus on genetics, reproduction, and animal welfare. Students will gain a comprehensive understanding of genetic engineering, transgenic animal models, and biotechnology’s role in medicine, agriculture, and sustainability.
Course Structure Lectures (5 ECTS, 40 hours)
The course covers the following topics:
A) Introduction to Biotechnology in Animal Production
Overview of biotechnology applications in animal production;
Fundamental morpho-physiological characteristics of farm animals;
Physiology and endocrinology of reproduction.
B) Genetic Engineering in Farm Animals
Transgenic Animals: Production and Applications:
Pigs as models for human disease research, organ transplantation, and environmental protection;
Cattle as biotechnological solutions for enhanced meat production, resistance to staphylococcal infections, and reducing milk protein allergies;
Teleost fish as models for cancer research.
Biopharming: Using the Mammary Gland as a Bioreactor:
Cattle producing therapeutic milk for human consumption;
Sheep and goats producing antimicrobial proteins for medical applications.
Recombinant Spider Silk Production:
Transgenic goats producing spider silk proteins in milk for medical and industrial applications.
C) Animal Welfare: Current Practices and Future Perspectives
Methods for evaluating and managing animal welfare;
Ethical considerations and sustainability;
D) Gut Microbiota and Animal Health:
The role of gut microbiota in animal health and productivity;
Strategies for microbiome management to enhance welfare and disease resistance.
Laboratory Practicals (1 ECTS, 12 hours)
Students will gain hands-on experience in key molecular biology techniques, including:
Gut Microbiota Analysis (0.25 ECTS, 3 hours):
Extraction of bacterial DNA for microbiome studies.
Gene Expression Analysis (0.25 ECTS, 3 hours):
Extraction of total RNA from animal tissue;
RNA integrity and quantity assessment;
Real-time PCR for gene transcript quantification.
DNA Fingerprinting (0.5 ECTS, 6 hours)
Part 1: Genomic DNA extraction, quantification, and purity assessment;
Part 2: Microsatellite DNA amplification via PCR, data collection, and analysis
Excursion
The course includes a field trip to an international center of excellence specializing in biotechnology applications in the food, nutraceutical, and pharmaceutical industries.
The course will take the form of face-to-face classes (5 ECTS), labs (1 ECTS), and a field trip. PowerPoint presentations that use images are extensively used in class to focus on visual learning, helping students grasp new information, concepts, and skills more effectively. Science videos supplement instruction, reinforcing key ideas and making complex topics easier to understand. Lab sessions are designed to actively engage students in hands-on work. Throughout the laboratory instruction, the professor and one or more assistants provide continuous supervision to ensure safety and support. Students are required to wear a lab coat during all laboratory activities. Participation in lab sessions is mandatory. Students with allergies or intolerances to reagents or other substances present in the lab must inform the professor before the laboratory sessions begin.
To avoid unnecessary delays, students must schedule an appointment via email (using an @uninsubria.it domain) for consultations with the professor. Additionally, the professor is available to meet with students immediately after each lecture.