METODI E TECNICHE DI BIOCHIMICA CLINICA 1
Students should have a good knowledge of basic Chemistry and have passed the exam of Chemistry the 1st semester.
Written exam, with questions relating to all the topics covered during the course, to define knowledge and understanding (25%), ability to apply knowledge and understanding (25%), independent judgment (10%), communication skills (20 %) and learning ability (20%).
Provide an overview of the basic principles of metabolism and metabolic effects of the main hormones.
Moreover, provide the knowledge of analytical techniques for use in clinical biochemistry. At the end of the course the student should be able to solve problems involving preparation of solutions and should know the principles of the analytical techniques most frequently used in clinical biochemistry
Basic principles: unit of measure, buffer solutions, pH, electrodes and pH measurement methods, quantitative measures, principles of biochemical-clinical analysis.
Chromatographic techniques: general principles, liquid chromatography, adsorption chromatography, partition chromatography, ion exchange, with molecular exclusion, affinity, gas-liquid.
Mass spectrometry: introduction, ionization, analyzers, detectors, protein analysis with the use of software programs.
Electrophoretic techniques: general principles, protein electrophoresis, nucleic acids, gel electrophoresis, capillary electrophoresis.
Principles and Techniques of Biochemistry and Molecular Biology, Authors: Keith Wilson e John Walker. Edited by Andreas Hofmann and Samuel Clokie.
An interdisciplinary approach will be applied in order to have a direct involvement of the students using frontal lessons and the critical analysis of the protocols used for the presented tests