MOLECULAR DIAGNOSTICS IN PATHOLOGICAL ANATOMY

Degree course: 
Corso di First cycle degree in Biomedical Laboratory techniques
Academic year when starting the degree: 
2020/2021
Year: 
3
Academic year in which the course will be held: 
2022/2023
Course type: 
Compulsory subjects, characteristic of the class
Seat of the course: 
Varese - Università degli Studi dell'Insubria
Credits: 
1
Period: 
First Semester
Standard lectures hours: 
60
Detail of lecture’s hours: 
Lesson (60 hours)
Requirements: 

It is necessary to have acquired basic knowledge of Biochemistry and Genetics

Oral exam aimed at verifying: the ability to organize knowledge; to critically reason on the study carried out; the quality of the exposure

Assessment: 
Voto Finale

The course aims to provide knowledge of the main molecular diagnostic tests currently used in Anatomic Pathology lab for the prevention, diagnosis and treatment of specific oncological diseases.

Introduction to molecular diagnostics in AP
Extraction of nucleic acids from fixed and paraffin-embedded tissues
Analysis of fragments using gel
electrophoresis or capillary electrophoresis
MLPA and MS-MLPA analysis
RT-PCR for gene mutation analysis
Gene mutation analysis using Sanger Sequencing
Gene mutation analysis with NGS

An interdisciplinary approach will be applied in order to have a direct involvement of the students using frontal lessons and the critical analysis of the protocols used for the molecular tests