MOLECULAR DIAGNOSTICS IN PATHOLOGICAL ANATOMY
It is necessary to have acquired basic knowledge of Biochemistry and Genetics
Oral exam aimed at verifying: the ability to organize knowledge; to critically reason on the study carried out; the quality of the exposure
The course aims to provide knowledge of the main molecular diagnostic tests currently used in Anatomic Pathology lab for the prevention, diagnosis and treatment of specific oncological diseases.
Introduction to molecular diagnostics in AP
Extraction of nucleic acids from fixed and paraffin-embedded tissues
Analysis of fragments using gel
electrophoresis or capillary electrophoresis
MLPA and MS-MLPA analysis
RT-PCR for gene mutation analysis
Gene mutation analysis using Sanger Sequencing
Gene mutation analysis with NGS
For consultation: “Principles of Molecular Diagnostics and Personalized Cancer Medicine” Autori: D. Tan e HT Lynch. Ed: Wolters Kluwer. ISBN/ISSN: 9781451131970
Other teaching materials: the slides used for the lessons are available on e-learning platform
An interdisciplinary approach will be applied in order to have a direct involvement of the students using frontal lessons and the critical analysis of the protocols used for the molecular tests