ANALYTICAL CHEMISTRY AND QUALITY CONTROL - Spectroscopy

Degree course: 
Corso di First cycle degree in BIOTECHNOLOGY
Academic year when starting the degree: 
2019/2020
Year: 
3
Academic year in which the course will be held: 
2021/2022
Course type: 
Supplementary compulsory subjects
Credits: 
2
Period: 
First Semester
Standard lectures hours: 
16
Detail of lecture’s hours: 
Lesson (16 hours)
Requirements: 

the student must have clear the concepts of molecular bonds, molecular weight, functional groups and polarity of the molecules, concepts provided in the previous courses of general and inorganic chemistry and organic chemistry

The exam includes written questions of exercises and theory

Assessment: 
Voto Finale

The principle aim of the course is to provide students with the skills to perform qualitative and quantitative analysis according to codified procedures, using the most common instrumental techniques. The main skills acquired will be:
• To know the principles, the instrumentation of the most common spectroscopic techniques to perform qualitative and quantitative analyzes;
• To know the chromatographic separation techniques and the different chromatographic retention mechanisms;
• To know the instrumentation for gas chromatography and for liquid chromatography;
• To know the principles of nuclear spectroscopy techniques and know how to analyze spectra related to this technique;
• To know the quality control standards for the validation of a method.

The spectroscopy module within the course of Analytical Chemistry aims to introduce problems related to NMR spectroscopy and to mass spectrometry, both techniques of considerable importance in the preparation of a future biotechnologist.

A) Nuclear magnetic resonance spectroscopy
- Principles of nuclear magnetic resonance
- Nuclear magnetic resonance for hydrogen
- Nuclear magnetic resonance for carbon in continuous wave
- Magnetic resonance in Fourier transform
- Two-dimensional magnetic resonance techniques
- Analysis of one-dimensional NMR spectra
- Molecular recognition exercises through NMR spectra
- B) Mass spectrometry
- Principles of mass spectrometry
- Instrumentation for mass spectrometry
- Analysis of mass spectrometry spectra

A) Nuclear magnetic resonance spectroscopy
- Principles of nuclear magnetic resonance
- Nuclear magnetic resonance for hydrogen
- Nuclear magnetic resonance for carbon in continuous wave
- Magnetic resonance in Fourier transform
- Two-dimensional magnetic resonance techniques
- Analysis of one-dimensional NMR spectra
- Molecular recognition exercises through NMR spectra
- B) Mass spectrometry
- Principles of mass spectrometry
- Instrumentation for mass spectrometry
- Analysis of mass spectrometry spectra

Lesson slide on E-learning
BOOKS: “Guida pratica alla interpretazione di spettri NMR”. Aut. Antonio Randazzo - Editore Loghia.
“Strumenti per il laboratorio chimico/biologico”. Aut. S. Polesello, A. Polesello, S. Guenzi, C. Roscioli – Editore: Tecniche Nuove (Mi)

Frontal lesson

Upon appointment via email using, preferably, the official student’s address: @studenti.uninsubria.it