METODI E TECNICHE DI BIOCHIMICA CLINICA 1
Students should have an optimum knowledge of basic Chemistry and have passed the exam of Chemistry the 1st semester.
Provide the theoretical knowledge of analytical techniques for use in clinical biochemistry. At the end of the course, the student should be able to solve problems involving preparation of solutions and/or an incorrect result obtained by a technique.
Basic principles: unit of measure, buffer solutions, pH, electrodes and pH measurement methods, quantitative measures, principles of biochemical-clinical analysis.
Chromatographic techniques: general principles, liquid chromatography, adsorption chromatography, partition chromatography, ion exchange, with molecular exclusion, affinity, gas-liquid.
Mass spectrometry: introduction, ionization, analyzers, detectors, protein analysis with the use of software programs.
Electrophoretic techniques: general principles, protein electrophoresis, nucleic acids, gel electrophoresis, capillary electrophoresis.
Principles and Techniques of Biochemistry and Molecular Biology, Authors: Keith Wilson e John Walker. Edited by Andreas Hofmann and Samuel Clokie.