ANALYTICAL CHEMISTRY 1 (MOD. A)
Knowledge of general and organic chemistry; basics of mathematics and physics. General Chemistry is a prerequisite for this exam.
The course will provide the theoretical background and the practical information related to analytical chemistry. The student will acquire knowledge regarding:
a) The analytical process, from the formulation of the analytical problem to the expression of the results;
b) Solving complex equilibria in solution;
c) Volumetric and gravimetric methods of analysis and their application;
d) Principles, hardware and application of the instrumental techniques presented during the course;
e) Quality in analytical chemistry and how to measure it;
f) Figures of merit of analytical methods;
g) Major operations in the analytical laboratory.
The skills the student is expected to acquire include:
a) Correct operation of the material in the analytical laboratory (glassware, balance, pHmeter, instrumentation)
b) Correctly applying a written method for the determination of an analyte (limited to the presented methodologies);
c) Problem solving ability for low difficulty analytical issues.
Module A
Introduction: analytical chemistry and its role in research and society.
Analytical protocol: definition of the problem, choice of the analytical method, sampling, sample treatment, determination, evaluation of the results (4 h).
Quality in analytical chemistry: quality control, quality assurance, validation, traceability, standard materials and standard analytical methods (2 h).
Gravimetric methods (2 h).
Electrochemical methods: potentiometry, conductimetry and amperometry (4 h).
Volumetric analysis: background, theory of neutralization, complexometric, redox and precipitation titrations; application of volumetric titrations (12 h).
Instrumental analysis: features of instrumental methods, block diagrams, analytical signal and calibration (2 h).
UV-visible and infrared (IR) absorption spectroscopy: theoretical aspects, instrumental apparatus and applications (8 h).
Chromatographic methods: theoretical aspects, stationary phases, detectors and applications (6 h).
Module B
General guidelines and mandatory behaviors for the safety in the lab - good laboratory practice
Introduction to descriptive and inferential statistics
Laboratory experiments:
Volumetric methods of analysis: acid-base titrations, complexometric titrations, redox titrations, precipitation titrations.
Instrumental methods of analysis: potentiometry – Ion Selective Electrodes (quantitative analysis), spectrophotometric analysis (UV-VIS and IR - quantitative and qualitative analyses), cromatographic analysis (HPLC - quantitative and qualitative analyses).
James F. Holler, Stanley R. Crouch, Fondamenti di Chimica Analitica, Edises 2015