Enzymology
Knowledge of Biochemistry and Organic Chemistry.
The course provides a description of the basics of enzymatic catalysis and factors which influence enzyme properties, as well as, protein folding. The course includes a discussion on the theoretical basis of enzyme catalysis and a quantitative analysis of enzyme reactions through a physical/mathematical approach. The course aims to provide the basis for a detailed knowledge of the mechanism of action of enzymes and their applications in biotechnology.
Module “Enzymology” Gianluca Molla: 4 CFU – 32 hours class (Lectures)
- Chemical kinetics
- Transition state theory
- Enzymatic catalysis
- Enzymatic kinetics
- Inhibition
- Multisubstrate reactions
- Rapid kinetics
- Allosteric enzymes
- Temperature effect
- pH effect
- ligand binding
- folding 1
- folding 2 (in vivo)
- Computational enzymology
Laboratorio (4 cfu, 32 ore):
Module “Laboratory of Enzymology” Gianluca Molla: 2 CFU – 32 hours lab (Practical)
- Exercitation focused on fundamental enzymological investigations
Slides of lessons: from the Elearning website
Lecture notes and papers (also for the laboratory): downloadable from Elearning site
Text book: Umberto Mura, "ENZIMI IN AZIONE" (EdiSES)